Journal: STAR Protocols

Article Title: Protocol to study secretome interactions using extracellular proximity labeling

doi: 10.1016/j.xpro.2024.103509

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Article Snippet: Polystyrene storage (roller) bottles , Corning , 8396.

Techniques: Recombinant, Modification, Saline, Electron Microscopy, Protease Inhibitor, Mass Spectrometry, Expressing, Plasmid Preparation, Control, Software, Imaging, Spectrophotometry, Suction Filtration, Pore Size, Membrane

Journal: mBio

Article Title: Role of Premycofactocin Synthase in Growth, Microaerophilic Adaptation, and Metabolism of Mycobacterium tuberculosis

doi: 10.1128/mBio.01665-21

Figure Lengend Snippet: mftD disruption increases growth fitness of M. tuberculosis in glucose-containing broth. All growth curve experiments were performed in 490-cm 2 polystyrene roller bottles with a liquid/air volume ratio of about 1:20 and rotation at 4 rpm. Cell densities (OD) were measured at 600 nm. (A) Growth of Δ mftD strain was higher than that of H37Rv and Δ mftD -Comp in Dubos medium. According to the Malthusian growth model, which is commonly used to estimate growth rate from during the exponential growth in liquid culture experiments, the estimated doubling time of the Δ mftD strain is 3.171 h compared to 4.41 h and 4.644 h for H37Rv and Δ mftD -Comp, respectively. (B to D) Growth of bacterial strains in modified m7H9 medium supplemented with 0.2% glucose alone (B), or in combination with cholesterol (0.01%) solubilized either in hot ethanol (cholesterol:EtOH) or hot DMSO (cholesterol:DMSO) (C and D). (E and F) Growth in cholesterol:EtOH (E) or cholesterol:DMSO medium (F) supplemented with 0.2% glucose and 3NP. Data presented in panels A to F are from three independent experiments performed in duplicate. Values shown are means ± standard deviations. (G and H) A representative growth curve of H37Rv and mutant derivatives in minimal medium containing cholesterol:EtOH and cholesterol:DMSO.

Article Snippet: Under each condition, bacterial cultures were grown in 490-cm 2 polystyrene roller bottles (Corning) with a liquid/air volume ratio of about 1:20 and rotation at 4 rpm at 37°C.

Techniques: Disruption, Modification, Mutagenesis